Applications for Streptavidin Coated Microplates
Streptavidin is a tetrameric protein of approximately 60 kDa in size. The streptavidin-biotin interaction is one of the strongest non-covalent bonds in nature. Streptavidin-coated surfaces serve as a universal binder for immobilization of any biotinylated molecule. Typically streptavidin plates are used for immobilization of capture antibodies, antigens or even small-size biotinylated molecules, which are hard to immobilize directly. Unlike proteins directly bound by passive adsorption, biotinylated proteins can be immobilized to streptavidin-coated surfaces with a lower risk of denaturation. Streptavidin coated plates are widely used in for example competitive immunoassays, reagents excess non-competitive immunoassays and oligonucleotide quantitation. Click picture to enlarge.