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Applications for Lanthanide Chelates

Lanthanide chelates can be used both in measurements from solution and measurements from surface. Kajo-chelates require a separate intensifying step: The lanthanide ion is released from Kajo-chelates into fluorescence intensifying solution. The Kajo chelates are optimal for high-sensitivity applications or assays performed with problematic sample matrices. For optimal performance use EFI-solution (europium fluorescence intensifier) with Kajo615 chelate. Loisto-chelates are intrinsically fluorescent and thus can be used as donors in homogeneous assays based on fluorescence resonance energy transfer (FRET), which is a widely used technique in high-throughput screening (HTS). Their long lifetime also enables background-minimizing time-resolved detection of the acceptor signal. Another application for Loisto chelates are assays based on direct surface measurement of lanthanide fluorescence. Click picture to enlarge.

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